Effect of 3,4-Toluenediamine on Output From In Situ Rat Brunner's Glands Pouches

SummaryDuodenal and gastric glandular mucosal damage have been observed 24 hr following single subcutaneous doses of 3,4-TDA in fed, unrestrained rats. 3,4-TDA significantly reduced secretion from in situ Brunner's glands pouches in pentobarbital anesthetized rats. The reduction in volume output with a definitely duodenal ulcerogenic dose of this compound was more than twice that observed with a minimally ulcerogenic dose, suggesting a correlation between the duodenal ulcerogenic and duodenal antisecretory activities of this compound. The animal model described in this communication should facilitate experimentation to establish the inhibitory effect of compounds on the output of protective fluids from the proximal duodenum.The authors thank Dr. T. G. Brown and Dr. A. S. Feigenbaum for their constructive criticisms of this manuscript, Dr. D. T. Drees for doing the histology, and Mrs. K. Littleton for secretarial assistance.