A fluorescein–gold nanoparticles probe based on inner filter effect and aggregation for sensing of biothiols

Abstract Cysteine (Cys), homocysteine (HCys) and glutathione (GSH) are sulfhydryl-containing amino acids known as biothiols being able to bind to gold nanoparticles (AuNPs) via sulfhydryl group, resulting in the aggregation of AuNPs. Owning to their inner filter effect, AuNPs can weaken or even quench the fluorescence of fluorescein. However, the introduction of biothiols to fluorescein-AuNPs leads to the recovery of fluorescein fluorescence. Thus, a simple and reliable turn on fluorescence method was developed for monitoring biothiols with fluorescein-AuNPs as a probe. Several factors, including AuNPs concentration, pH value and incubation time, which might influence the fluorescence reclamation of fluorescein-AuNPs probe, were optimized by taking Cys as an example at room temperature. Under the optimal conditions, sensitive sensing of Cys, HCys and GSH was achieved. The detection limits for Cys, GSH, and HCys were 0.027, 0.023, and 0.030μΜ, respectively. This method was used to the determination of Cys in human serum samples with high precision and accuracy, indicating the potential of the method in practical applications with simple operation, good accuracy and high sensitivity.