Quick Preparation of Total DNA from Plants

A fast and simple way to isolate total DNA from up to 100mg of plant tissue was developed in the present paper. By using the DNeasy Plant Mini Kit, high quality of total DNA from plant was yielded in less than 1 hour. Purification required no phenol or chloroform extraction and involved minimal handling. This made the kit ideal for simultaneous processing of multiple samples. Plant DNA isolated with this method could be completely digested by restriction endonucleases, and was ready for use in all kinds of downstream applications including PCR, blotting, analysis of RAPD, AFLP and RFLP.