Micropropagation de deux variétés de papayer ( Carica papaya L.) à l’IPR/ IFRA de Katibougou, Mali

Cette etude, achevee en septembre 2014, est axee sur : la production de plantules de Solo n°8 et Bluestem pour l’obtention d’explants, la desinfection d’explants, la realisation de 2 essais d’initiation et de proliferation, et d’un essai d’enracinement de pousses en randomisation totale. L’evaluation de l’etude a porte sur l’efficacite de la formule de desinfection d’explants, la performance des milieux d’initiation et de proliferation de deux essais comportant respectivement 6 milieux (essai 1) et 4 milieux (essai 2), tous associes a 2 types d’explants (bourgeons apicaux et axillaires), la performance de 3 milieux d’enracinement. Les resultats obtenus ont revele que le milieu MS + 0,5 mg/l de BAP associe aux bourgeons apicaux a donne les nombres de feuilles et de pousses les plus eleves, ainsi que le meilleur delai de reprise (7 jours), le milieu MS + 0,5 mg/l BAP + 2 mg/l d’AIB associe a la variete Solo no8 a ete plus favorable a l’enracinement de pousses (33%), en nombre de racine (1), en longueur de racines (0,8 cm) par vitro plant, et en delai d’emission de racines (18 jours). Les deux varietes se valent en initiation et proliferation. Mais pour l’enracinement, la variete Solo no8 a ete la meilleure. © 2017 International Formulae Group. All rights reserved. Mots cles: AIB, BAP, bluestem, bourgeons, solo n °8 English Title: Micropropagation of two varieties of pawpaw trees ( Carica papaya L.) at IPR /IFRA of Katibougou, Mali English Title This survey, finished in September 2014, is focused on the production of two papaya varieties Solo n°8 plantlets and Bluestem for the obtaining of explants, the disinfection of explants, the realization of two (02) essays of initiation and proliferation, and of a test of shoot rooting in total randomization. The assessment of the survey carried on the efficiency of the formula of explants decontamination, the medium performance of initiation and proliferation of two tests including 6 mediums respectively (test 1) and 4 mediums (test 2), altogether to 2 types of explants (ending buds of plant and axillaries buds), the performance of 3 rooting mediums. The obtained results revealed that the MS middle + 0,5 mg/liter of BAP partner to ending buds gave numbers of leaves and the most elevated shoots, as well as the best time limit of retaking (7 days), the M S medium + 0,5 BAPS mg / liter + 2 mg / liter of AIB partner to the Solo variety n o8 were more favorable to the  rooting of shoots (33%), in number of root (1), in length of roots (0,8 cm) by plantlet, and in emitting time of root (18 days). The two varieties are the same in initiation and proliferation. But for rooting, the Solo variety no8 was the better. © 2017 International Formulae Group. All rights reserved. Keywords: AIB, BAP, bluestem, buds, plant height, solo n °8