Genetic diversity analysis on rhizosphere soil microbial population of Panax ginseng and Panax quinquefolium by RAPD.

Objective To study rhizosphere and non-rhizosphere soil microbial population genetic diversity of Panax ginseng and P.quinquefolium with different growing years.Methods Eighteen P.ginseng and P.quinquefolium rhizosphere and non-rhizosphere soil samples were collected from Fusong county of Jilin Province,and genetic diversity of microbial population construction were analyzed by RAPD.Results Cultivation times have great influence on microbial population construction of rhizosphere and non-rhizosphere soil.Also we found that there were differences between soil microbial population construction of P.ginseng and P.quinquefolium.Furthermore,rhizosphere effect of plants on soil microbial population construction was also found.Conclusion Directional selection pressure that root secretions of P.ginseng and P.quinquefolium to soil microorganisms is one of the main drives that resulted in the change of microbial population genetic diversity,and microbial population construction change in P.ginseng and P.quinquefolium cultivated soil is the key factor for soil ecological function turbulence and the development of continuously cropping obstacle.