CDNA Cloning and Sequence Analyses of Six Chicken Z鄄Linked Genes from Peking Gecko(Gekko swinhonis)

We sequenced partial cDNA sequences of the homologues of six avian Z鄄linked genes from Gekko swinhonis by using the RT鄄PCR approach. Comparing with those obtained from G. hokouensis,the lengths of all these fragments were identical at both nucleotide and amino acid levels except the 5忆 region of CHD1,in which there had a deletion of 3 bps;fur鄄 thermore,sequence homologies were very high (97% - 100%). When compared with other exsiting sequences from Squamata,Testudines and Crocodylia,varying degrees of similarity were found in sequences of eight fragments from six genes. GHR showed the lowest homology,which was concordant with the relatively high value of 棕(Ka/ ks). Cloning and chromosomal location of the sex鄄chromosome linked genes will be helpful to elucidate the origins and evolutionary processes of different sex chromosomes in geckos or even in higher vertebrates.