Protection by tetrahydroxystilbene glucoside against neurotoxicity induced by MPP+: The involvement of PI3K/Akt pathway activation

Abstract Oxidative stress plays an important role in the pathogenesis of Parkinson's disease (PD). 2,3,5,4′-tetrahydroxystilbene-2-O-β- d -glucoside (TSG), which is an active component of the rhizome extract from polygonum multiflorum, shows potent antioxidant properties. In this paper, the neuroprotective effects of TSG on 1-methyl-4-phenylpyridinium (MPP + )-induced apoptosis in PC12 cells were investigated. Pretreatment with TSG markedly attenuated MPP + -induced loss of cell viability and release of lactate dehydrogenase (LDH), and reduced MPP + -induced apoptotic cell death in a dose-dependent manner. The anti-apoptotic effects of TSG were probably mediated by the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway since TSG failed to rescue cells from MPP + injury in the presence of the PI3K inhibitor, LY294002. These results indicate that TSG affords a significant neuroprotective effect against MPP + -induced damage and apoptosis in PC12 cells. The PI3K/Akt signaling pathway might be involved in the TSG-mediated anti-apoptotic effects.