Fusaristatins D–F and (7S,8R)-(–)-chlamydospordiol from Fusarium sp. BZCB-CA, an endophyte of Bothriospermum chinense

Abstract Three new lipodepsipeptides, fusaristatins D–F (1–3) and a new α-pyrone derivative, (7S,8R)-(–)-chlamydospordiol (5), together with eight known compounds (4, 6–12) were obtained from solid rice cultures of Fusarium sp. BZCB-CA, an endophyte of the Chinese medicinal plant, Bothriospermum chinense. The planar structures of the new metabolites (1–3, 5) were established by spectroscopic techniques (1D/2D NMR and HRESIMS). Marfey’s method was applied to determine the absolute configuration of 1, while the absolute configuration of 5 was determined by single-crystal X-ray crystallography analysis in addition to Mosher’s method. Crystallographic data of inflatin C (7) are also supplied here for the first time. In cytotoxicity assays, rubrofusarin (8) showed a moderate effect on the lymphoma cell lines L5178Y, Ramos and Jurkat, with IC50 values of 7.7, 6.2 and 6.3 μM, respectively, while the remaining compounds were inactive. When subjected to antibacterial assay, only lateropyrone (9) exhibited good to weak activity against a panel of Gram-positive bacteria including drug-resistant strains with MICs ranging from 3.1 to 25 μM.