Cloning and Analysis Expression Patterns of GhSPS1 Gene in Cotton

【Objective】 The GhSPS1 gene was cloned and analyzed the expression patterns under abiotic stresses treatments.In different tissues and at fiber developmental stages,transcriptional patterns of the gene were also detected.【Method】 The DNA and cDNA sequence of GhSPS1 were cloned from cotton by genome-walking and over-lap PCR,respectively.The expression patterns of GhSPS1 were analyzed by using semi-quantitative PCR when the cotton seedlings were treated with abiotic stresses.The characteristics of four genes,GhSPS1,GhSUS3,GhINV and GhCESA4,were examined by real-time quantitative PCR at fiber developmental stages.【Result】 Comparison GhSPS of DNA and cDNA sequence,the results showed that GhSPS1 is 4 545 bp,with 3 108 bp ORF encoding 1 035 amino acids,include 10 introns and 11 extrons.The results of semi-quantitative PCR exhibited that the mRNA levels of GhSPS1 increased when the cotton seedlings were treated with ABA and low temperature.In high temperature treatment,the expression of GhSPS1 decreased firstly,and then followed with a little increase.Analysis of the genes expression patterns by real-time quantitative PCR showed that GhSPS1 and GhSUS3 expressed in all tissues,but the highest expression was in 0 DAF ovules,3 DAF and 18 DAF fibers.On the contrast,GhINV transcripts were expressed higher in 3-15 DAF fibers than in other tissues.And GhCESA4 transcripts had the highest expression level in 18 DAF fibers.【Conclusion】 GhSPS1 belongs to SPSA family,which can be induced by using ABA,low and high temperature treatments.The expression of the gene might have close relationship with initial fiber development and secondary wall thickening.