Identificação de Hb AB2 em dois caucasianos da região Amazônica por procedimentos eletroforéticos e cromatográficos Identification of Hb AB2 in two Caucasian of the Amazonian region by electrophoresis and chromatography

Abstract Hemoglobin B2, variant of δ globin chain, presentsan increased frequency in African and afro-descendant populations but is considered rare inCaucasians. It does not present physiopathologyeffects in heterozygosis and it has been spread aroundthe world by the founder effect. In this paper we reporton the identification of Hb B2 in heterozygosis, by theassociation of electrophoretic and chromatographyprocedures, in two Caucasian women from Acre andPara States, Brazil, which are endemic areas forMalaria. Key words: δ globin variant; Hb B2; Hb A2 variant. Referencias Bibliograficas 1. Spurdle AB, Krause A, Ramsay M, Jenkins T. The high frequency ofthe B2 variant in the Herero population: a founder efect?Hemoglobin 1994; 18: 317-234.2. Schiliro G, Russo-Mancuso G, Dibenedetto SP, et al. Six rare hemoglobinvariants found in Sicily. Hemoglobin 1991;15(5):432-7.3. Bannani M, Mombo LE, Chaventre A, et al. Origin of Hb A2’ (HbB2) [d16 (A13) Gly®Arg (GGC®CGC). Hemoglobin 2003;27(2):105-10.4. Honig GR, Adams JG. Human Hemoglobin Genetics. Springer-VerlagWien New York 1986.5. Domingos ACB, Viana-Baracioli LMS, Bonini-Domingos CR. Iden-tificacao de variantes de hemoglobinas em doadores de sangue.Rev Bras Hematol e Hemoter 2004;26(1):57-9.6. Souza WC, Torres FR, Salvador AR, et al. Avaliacao de Hb A2 e HbF em doadores de sangue de regiao malarigena da Amazonia Orien-tal brasileira por HPLC. Rev Bras Hematol e Hemoter 2003; 25(4):263-6.Figura 2. Perfil cromatografico obtido pelo equipamento Variant (Bio-Rad) de HPLCem doadores do Para (A) e Acre (B). As setas cheias indicam a fracao de Hb A2reduzida e as pontilhadas a fracao de Hb B2.Avaliacao: Editor e dois revisores externos.Conflito de interesse: assessor cientifico da Bio-Rad para a linha deBiologia Molecular (Life Science) desde 2001Recebido: 25/04/05Aceito apos modificacoes: 18/05/05