The Use of Kidney Biopsy of Broodstock Steelhead Trout (Oncorhyncus Mykiss) to Determine the Status of Bacterial Kidney Disease Infection

of other staining methods, including immunohistochemical techniques. Mounting small biopsy specimens on cucumber slices allowed for easier manipulation and visualization of tissues. This procedure enabled specimens that were too small to manipulate to remain intact during processing and embedment. In addition, the cucumber slice served as an aid for proper orientation of the specimen. Albumin was not used for adherence of the specimen to the cucumber, as has been reported. Utilization of the end paper during processing ensured adhesion of the specimen to the cucumber and serves as a small field to work in while embedding. This procedure can be employed for virtually any tissue specimen for which proper orientation or loss of a specimen during processing is a concern. Acknowledgements. We thank Robin Olmscheid and Tom