PROSPECÇÃO DE GENES EM BIBLIOTECAS DE cDNA GENE PROSPECTION IN cDNA LIBRARIES

Gene prospection is one of the current challenges for science. Molecular biologists and Bioinformatics need to analyze a large amount of information. Gene prospection still constitutes an area to be explored, mainly in eukaryotes. It involves the combination of bioinformatics, with in silico analyses, and molecular biology with the construction and screening of cDNA libraries. The strategy of gene prospection begins with the construction of cDNA libraries, followed by a search for homology in public databases. Soon afterwards, conserved regions are identified on the selected sequences, throughout multiple alignments. With this information, degenerate primers may be constructed for cDNA screening, which are analyzed to screen one or more genes, using labeled probes or DNA amplification by PCR. The spectacular progress of bioinformatic in the last few years, and the growing computational capacity and speed access on the Internet, helped to accomplish new analyses. The development of methodologies and algorithms that allow elucidating all genes and proteins combinations is the new border of the bioinformatics and the molecular biology.