TOP-GAN: Label-Free Cancer Cell Classification Using Deep Learning with a Small Training Set.

We propose a new deep learning approach for medical imaging that copes with the problem of a small training set, the main bottleneck of deep learning, and apply it for classification of healthy and cancer cells acquired by quantitative phase imaging. The proposed method, called transferring of pre-trained generative adversarial network (TOP-GAN), is a hybridization between transfer learning and generative adversarial networks (GANs). Healthy cells and cancer cells of different metastatic potential have been imaged by low-coherence off-axis holography. After the acquisition, the optical path delay maps of the cells have been extracted and directly used as an input to the deep networks. In order to cope with the small number of classified images, we have used GANs to train a large number of unclassified images from another cell type (sperm cells). After this preliminary training, and after transforming the last layer of the network with new ones, we have designed an automatic classifier for the correct cell type (healthy/primary cancer/metastatic cancer) with 90-99% accuracy, although small training sets of down to several images have been used. These results are better in comparison to other classic methods that aim at coping with the same problem of a small training set. We believe that our approach makes the combination of holographic microscopy and deep learning networks more accessible to the medical field by enabling a rapid, automatic and accurate classification in stain-free imaging flow cytometry. Furthermore, our approach is expected to be applicable to many other medical image classification tasks, suffering from a small training set.