Five-conjugate testing card for detecting beta-stimulant drugs and preparation method thereof

The invention relates to a five-conjugate testing card for detecting beta-stimulant drugs and a preparation method thereof and belongs to the technical field of beta-adrenergic receptor stimulant detection. A test strip is arranged in a casing of the testing card which is composed of a polyvinyl chloride (PVC) glue board, a sample pad, a colloidal gold combination pad, a coated film and absorbent cotton. A colloidal gold film is a glass cellulose film which contains colloidal gold markers of a clenbuterol monoclonal antibody, a ractopamine monoclonal antibody, a salbutamol monoclonal antibody, a phenylethanolamine A monoclonal antibody and a terbutaline monoclonal antibody. The coated film is a nitrocellulose film, wherein five detection lines T and a quality control line C are arranged on the coated film, and the quality control line C is coated with a rabbit anti-mouse immunoglobulin G (IgG) antibody. The five-conjugate testing card can simultaneously detect out clenbuterol, ractopamine, salbutamol, phenylethanolamine A and terbutaline, and is simple, convenient and fast in method, and accurate in result.