Isolation and Determination of Corosolic Acid in Lagerstroemia specious Leaves

采用2次制备TLC分离法,从大叶紫薇叶(Lagerstroemia specious L.)中提取了corosolic acid纯品.通过IR,MS,1H NMR和13CNMR谱图鉴定了corosolic acid的结构,用高效液相色谱法(HPLC)测得corosolic acid 的纯度为98.8%,并且采用HPLC法对大叶紫薇叶中corosolic acid含量进行了测定,测定方法准确性高、重现性好.