Determination of the Contents of Three Components of Ibuprofen,Pseudoephedrine Hydrochloride and Chlorphenamine Maleate Capsule by HPLC

Objective To establish a method for determination of the contents of ibuprofen,pseudoephedrine hydrochloride and chlorphenamine maleate capsule.Methods HPLC was undertaken using Zorbax-C18(4.6 mm×250 mm,5 μm)with a flow rate 1 mL·min-1 and detected at 259 nm and 265 nm.Mobile phase was consisted of methanol(6 mmol·L-1),sodium lauryl sulfate(0.025 mol·L-1),potassium dihydrogen phosphate and triethylamine(75:20:20:0.25,pH=3.25).Results The linearity between concentration and absorption was found in the range of 100.6-704.2 μg·mL-1 for ibuprofen,50.0-600.0 μg·mL-1 for pseudoephedrine hydrochloride and 5.02-50.20 μg·mL-1 for chlorphenamine maleate.The average recycle rate of ibuprofen,pseudoephedrine hydrochloride and chlorphenamine maleate was 99.7%,99.1% and 99.1%,and RSD was 0.71%,0.73% and 0.71%(n=5),respectively.Conclusion HPLC was a reliable,accurate and suitable method for the determination of the ibuprofen,pseudoephedrine hydrochloride and chlorphenamine maleate in the capsuley.