Effects of experimental xylitol varnishes and solutions on bovine enamel erosion in vitro

This in vitro study aimed to analyze the effects of application of xylitol varnishes and solutions to protect against enamel erosion. Twelve bovine enamel specimens were pre-treated with 5% NaF-Duraphat varnish, 10% xylitol varnish, 20% xylitol varnish, placebo varnish, 5% NaF solution, 10% xylitol solution or 20% xylitol solution. The varnishes and solutions were applied for 6 h and 1 min, respectively. Controls remained untreated (n = 12). Specimens were then subjected to erosive demineralization (Coca-Cola, 4 ! 90 s/d) and remineralization (artificial saliva, 2 h) cycling for 10 days. After 5 days, the varnishes and solutions were reapplied. After reapplication, two specimens per group were analyzed by SEM. Enamel loss was measured profilometrically after the 5th and 10th days. Data were then analyzed statistically by ANOVA and Tukey's post-hoc test (n = 10, P < 0.05). After the 5th day, all varnishes and 20% xylitol solution significantly reduced the enamel loss when compared to the placebo varnish/control. After 10 days of erosive pH cycling, both xylitol varnishes and solutions significantly reduced the enamel erosion when compared with the control. However, 10% xylitol solution produced a smooth layer on eroded enamel and significantly reduced the enamel erosion when