Study on the correlation among quantification of HBV-DNA and HBeAg,anti-HBe in hepatitis B carriers

Objective To better understand the duplication of hepatitis B virus (HBV) in order to improve clinical diagnoses and treatments via quantitative measurement of HBV-DNA and comparison of correlation of HBV-DNA with HBeAg and anti-HBe. Methods For 883 hepatitis B patients with positive HBsAg, HBV-DNA was measured by COBAS AMPLICOR HBV MONITOR?reagent and COBAS AMPLICOR quantitative PCR instrument. Microparticle enzyme immunoassay analysis (MEIA) was then carried out with fully automatic enzyme immunoassay analysis instrument made by Abbott Axsym from the U.S. to measure HBeAg and anti-HBe. Correlation was analysed by SPSS. Results (1) Positive correlation between 690 HBV-DNA positive and HBeAg positive with r = 0.505 ( P 0.01) was found with mean values as:HBV-DNA:7.12×1012 copies/ml;HBeAg:218.31 S/CO. HBV-DNA:104 copies/ml, HBeAg: 104 S/CO; HBV-DNA: 105-108 copies/ml, HBeAg: 112 S/CO; HBV-DNA: 109-1015 copies/ml, HBeAg: 252 S/CO. (2) No correlation was found between 193 HBV-DNA and anti-HBe + with r= -0.052(P= 0.4770.05) with Mean: HBV-DNA: 8.0×1010 copies/ml anti-HBe: 0.18 S/CO. Conclusion HBV-DNA and HBeAg appeared to have had linear correlation, showing that HBeAg 100 S/CO,HBV-DNA104 copies/ml and hepatitis B virus were reproduced. However,HBV-DNA did not show linear correlation with anti-HBe as HBeAg negative and anti-HBe positive, the level of hepatitis B viral replication decrease slightly. But the virus load is still high. Infectivity can not neglect.