CHEMICAL STUDY OF ADENOCALYMMA AXILLARUM, AND EVALUATION OF CITOTOXICITY AND LEISHMANICIDAL ACTIVITIES

Purpose: The leaves ethanol extract from Adenocalymma axillarum (K. Schum.) L.G. Lohmann (Bignoniaceae) presented leishmanicidal activity and was not cytotoxic in human lung fibroblast cells. Thus, the aims of this study were chemical fractionation of A. axillarum bioactive crude extract and evaluation of the leishmanicidal and cytotoxic activities. Methods: The bioassays were conducted against Leishmania amazonensis amastigotes forms, and the cytotoxicity was assessed in normal human lung fibroblasts cell line (GM07492A). The bioactive extract was subjected to high-performance liquid chromatography-diode array detector and high-resolution mass spectrometry (HPLC-DAD-HR-ESI/MS). The extract was also purified by liquid-liquid partition. The obtained fractions were evaluated in the biological assays. So, the bioactive EtOAc fraction was subjected to silica ODS column, furnishing 5 fractions. The fraction 1 was purified using Sephadex LH-20 column. Results: The pseudo-molecular ions [M-H] - were observed at m/z 421.1322, m/z 463.0847,  and m/z 477.1008 in HR-ESI/MS spectra. These ions suggested the presence of 6-β-hydroxypolamiide, hyperin, and cacticin in the extract. The detected compounds have been previously isolated from Adenocalymma peregrinum . Conclusion: Further studies are underway in order to purify these compounds from the obtained fractions with the aim of isolating the bioactive compounds since those fractions have potential biological activity.