Loss of Immunoreactivity of Human Serum Parathyroid Hormone by the Action of Rat Kidney Enzyme, Preferentially Hydrolyzing Parathyroid Hormone

Acetone powder of rat kidney was extracted with0.15 m KCl containing 5 mM tris-Cl and 15% glycerol. The fraction, precipitated by 35-75% saturated ammonium sulfate, was chromatographed on a Biogel P-3