Demonstration of glutamate decarboxylase-immunoreactive neurones in cultures of rat substantia nigra

Abstract Glutamate decarboxylase was immunohistochemically localized in primary cultures of newborn rat substantia nigra. The reactivity was restricted to the cytoplasm of a few neurones. The findings suggest that γ-aminobutyric acid (GABA)-synthesizing cells are present in substantia nigra and suggest that some nigral neurones may use GABA as a transmitter.