血友病A, B患者血漿中のHIV-2抗体: 合成ペプチドイムノアッセイ及びPCR法を用いての検索

The antibodies against HIV-2, the alternative etiologic agent for acquired immunodeficiency syndrome (AIDS), were evaluated on a total of 121 Japanese hemophiliacs (106 hemophilia A, and 15 hemophilia B), using a combination of enzyme-immunoassay (ELAVIA-2), western blotting (LABBLOT 2), and peptide immunoassay (PEPTILAB1-2) provided from Pasteur-Sanofi, France. The twenty eight patients of hemophilia A (26%) and the three of hemophilia B (20%) showed positive results by ELAVIA-2. PEPTILAB 1-2 test specified that only one patient (case X) with hemophilia A has a double reaction to HIV-1 and HIV-2 specific peptides. LABBLOT 2 also demonstrated the immunoreactivity of case X to HIV-2 proteins; p26, gp36, gp105, and gp140. Case X was further examined using polymerase chain reaction (PCR) method to detect proviral DNA sequences of HIV-1 and HIV-2, and finally the possibility of HIV-2 infection was ruled out confirming true HIV-1 infection.