Antioxidant activity of alstonia Angustifolia ethanolic leaf extract

In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaves has a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 µM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 µM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 µM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 µg/ml and 39.60 µg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 µg/ml compared to standard ascorbic acid 466.56 µg/ml. Thus, the study suggests that A. angustifol...