Immunomorphologic evaluation of the reserves of endotoxin binding by polymorphonuclear leukocytes

It is shown that leukocytes binding endotoxin of gram-negative bacteria via Fe-receptor mediated mechanism can be detected in blood smears after the treatment with antibodies to Re chemotype glycolipid conjugated with horse-radish peroxydase. After pretreatment of blood smears by a solution of endotoxin and then by conjugated antibodies to Re glycolipid some leukocytes binding endotoxin in vitro can be detected. It means that some reserves of endotoxin binding by leukocytes can be estimated by this immunomorphological method. The presence of such reserves is characteristic for healthy people and animals. Such reserves were enhanced in animals immunized with heated vaccine from Re Salmonella mutant. The reserves of endotoxin binding by leukocytes are absent in patients with salmonellosis, dysentery, meningitis, viral hepatitis A and B, peritonitis.