Expression protein detecting bovine parainfluenza virus 3 antibody and ELISA kit

The invention relates to expression protein detecting bovine parainfluenza virus 3 antibody. The expression protein is recombinant escherichia coli BL21/pGEX-NP2 capable of expressing bovine parainfluenza virus 3 NP gene C' end rNc protein, and is characterized in that rNc protein is 391st-500th site amino acid coded by 330bp at the coding region of NP gene C' end, the recombinant plasmid of the recombinant escherichia coli is a prokaryotic expression plasmid expressing the NP gene C' end coding region as 330 base. the expression protein is a recombinant escherichia coli strain, is capable of specifically expressing bovine parainfluenza virus 3 NP gene C' end rNc protein as an antigen, and is capable of effectively performing diagnosis after virus infection and vaccine immunization.