Reference Volume Generation for Subsequent 3D Reconstruction of Histological Sections

Anatomical reference brains are indispensable tools in human brain mapping, enabling the integration of multimodal data or the alignment of a series of adjacent histological brain sections into an anatomically realistic space. This study describes a robust and efficient method for an automatic 3D reconstruction of blockface images taken from postmortem brains during cutting as a prerequisite for high-quality 3D reconstruction of brain sections. The refinement technique used in this registration method is applicable for a broad range of pre-registered histological stacks.