Isolation, identification, cloning and phylogenetic analysis of the micro algae Prorocentrum minimum (Pavillard) J. Schiller 1933 Isolated from the Bay of Callao - Peru

Harmful Algal Blooms (HAB) or Red Tides are water discolorations visible to the nakedeye due to the proliferation of one or more planktonic microorganisms such as microalgae. Thesecan reach high levels and produce adverse effects on human health, as well as cause damage toother marine organisms near the coast. For this reason, there was interest in isolating, identifyingand making a phylogenetic study of the species Prorocentrum minimum found in the Bay ofCallao, Peru. Several samples of seawater were taken for their subsequent morphologicalidentification, and the technique of purification of the microalgae was carried out. At the sametime, a standard of Prorocentrum minimum was acquired from the Provasoli- Guillard NationalCenter for Culture of Marine Phytoplankton (CCMP), USA. It was carried out the growth curvefor the concentration of microalgal density, later it was carried out the DNA extraction and themolecular phylogeny from the sequences of the ribosome LSU rRNA subunits of the speciesProrocentrum minimum. P. minimum and P gracile were identified, which were close epibenthicspecies because they were more recent evolutionarily. Likewise, to confirm the presence of somemarine biotoxin absence in Prorocentrum minimum culture, it was established a quantitativeanalysis of the animal's dose response in the mouse bioassay. It was possible to obtain thepurification of Prorocentrum minimum culture and to standardize the work protocol; it wasconfirmed the phylogeny of microalgae species. It was not possible to obtain DSP toxin frommicroalgae culture.