Inhibition of telomerase activity of human colon cancer cell line by compound traditional Chinese medicine kang ai fang

目的了解中药复方抗癌方(KAF)对人结肠癌细胞端粒酶活性的影响.方法将HC8693细胞株作为靶细胞,用MTT试验测定细胞毒作用,用TRAP半定量法测定端粒酶活性.结果 KAF对HC8693的半数致死量(LD50)为25.4%;染毒后,端粒酶活性降低(5%KAF组:4676.7±478.1;10%KAF组:3581.3±632.4;空白对照组:6003.3±337.9.P＜0.05或P＜0.01),去除KAF24小时后,端粒酶活性仍维持在较低水平(4495.0±423.9,P＜0.05).结论 KAF能抑制人结肠癌细胞端粒酶活性.