Amphoteric ion hydrophilic chromatographic stationary phase and preparation method thereof

The present invention relates to a liquid chromatography stationary phase, which is bonded with the end of a zwitterionic functional group having the formula: wherein SiO2 is silica gel, an amino acid, polypeptide, or protein is, n = 0 ~ 4, R2 is vinyl or ethynyl, X + represents an amino cation, Y- represents a carboxyl group anion. The present invention also provides a preparation method of the liquid chromatography stationary phase, by means of horizontal polymerization of the alkenyl or alkynyl group chemically bonded to the silica surface of the obtained alkenyl or alkynyl silica gel, and then with a mercapto group zwitterionic compound via sulfhydryl The reaction can click zwitterionic hydrophilic chromatographic stationary phases prepared. Novel structure of the present invention provides a stationary phase having zwitterionic characteristics and a good hydrophilicity, very suitable as a new class of stationary phase hydrophilic interaction chromatography, it can be used in various types of sample separation.