Repression activity of recombinant oncostatin M on proliferation and migration of tumor cells

AIM: To investigate the inhibition ability of recombinant Oncostatin M(OSM) to proliferation and migration of tumor cells. METHODS: Expressed and purified human OSM from recombinant E.Coli, and assayed its pharmacology activity by a series of experiments in vitro to metastatic human lung cancer cell line 95-D including MTT assay, clone formationassay in soft agar, cell attachment assay, cell migration assay and invasion cup assay. RESULTS AND CONCLUSION: rhOSM displayed strong activity of inhibiting the proliferation and migration of 95-D. Prokaryoticly expressed hOSM has potential clinical value to lung cancer.