Low‐cost biotransformation of glycerol to 1,3‐dihydroxyacetone through Gluconobacter frateurii in medium with inorganic salts only

: Existing dihydroxyacetone (DHA) production practices require the use of yeast extracts, leading to relatively high production costs. This study explores the use of low-cost media comprising glycerol, inorganic salts and Gluconobacter frateurii BCC 36199 in the production of DHA. The medium components are also quantitatively optimized. Regression models describing the linear correlations between the nutrient concentrations and the generated DHA concentration (p), and between the nutrient concentrations and the yield (ysp ) are developed. Under the optimal conditions according to our regression models, the highest values for p and ysp are 29·36 g l-1 and 97·86% g g-1 respectively. Quantitatively, this study shows positive effects of inorganic salts and adverse effects of excessive amounts of glycerol on DHA production. In particular, the results suggest that low levels of biomass production lead to high levels of DHA production. Consequently, the media containing inorganic nitrogen source from (NH4 )2 SO4 lead to higher yields than organic media containing yeast extract. This study has identified an optimal, low-cost, minimal medium that can effectively enhance DHA production. SIGNIFICANCE AND IMPACT OF THE STUDY: This study illustrates the advantages of inorganic nutrients supplementation over organic nutrient supplementation for a lower media cost and a higher dihydroxyacetone (DHA) production yield through Gluconobacter frateurii BCC 36199 cultivation. The study found that the use of media that contain only glycerol and inorganic salts enhanced DHA production (DHA-Prod) while keeping the production of bacterial biomass at a sufficient level. Most of the starting material, that is, glycerol, is converted into DHA, which is the target of the production process. The cost of the nitrogen supplement in the DHA-Prod process may be reduced by up to 80% through the use of the inorganic culture medium that has been developed in this study.