An Effect of Insulin on Cyclic Adenosine 3': SMonophosphate

SUMMARY Homogenates of rat fat cells were separated into three fractions by centrifugation: Pi (sedimented at 10,000 x g for 7 min), Pz (sedimented from the 10,000 x g supernatant after 20 min at 100,000 x g), and S (the 100,000 x g supernatant). All fractions contained adenosine 3’:5’-monophosphate (CAMP) phosphodiesterase activity. The S fraction was enriched in higher