Bactericidal Capability and Respiratory Burst Characteristics of the Murine Macrophage Cell Line RAW264.7.

Abstract : The murine macrophage RAW264.7 cell line was used to establish assays of phagocyte functional status as part of an in vitro toxicity testing program. The variables included antimicrobial activity (toward S. epidermidis) and the respiratory burst (oxygen consumption, superoxide anion radical and hydrogen peroxide release) in response to 12-0-tetradecanoyl-13-phorbol acetate (TPA) and opsonized zymosan. The cells had potent antimicrobial activity. The magnitude of the respiratory burst in oxygen consumption was highly dependent on culture conditions. Additional keywords: cell culture bactericidial assays; bactericidal potency; Stanford cells.