Layer-by-Layer DNA film synthesis via branched hybridization

A new Layer-by-Layer (LbL) DNA film synthesis, where the driving force is the natural DNA hybridization, is presented in this work. The DNA films were synthesized via a branched mechanism and do not include any chemical binders between each layer as it is the case for film obtained by other synthesis paths. Kinetics of film formation were monitored by mass measurements with an electroacoustic network analyzer. The presented synthesis is a pathway to new DNA structures which can be used in biotechnologies and is complementary to those obtained by other LbL techniques.