Generation of green fluorescent protein vector transformed Paecilomyces lilacinus Strains

Paecilomyces lilacinus is one of the most important biocontrol fungi and has been applied extensively for controlling root-knot nematodes. The protoplast/PEG/CaCl2 method was used to generate green fluorescent protein (GFP) transformants of two strains ofPaecilomyces lilacinus (strain 20-7 and 618). Two transformed strains were successfully generated and identified by fluorescence microscopy. Transformation was confirmed by recovery, cloning and sequencing of polymerase chain reaction (PCR) products and Southern blot analysis. Two stably transformed strains were isolated (20-7 and 618 strain). GFP transformants of Paecilomyces lilacinus provide useful resources for elucidating the mechanism of controlling root-knot nematodes.   Key words: Paecilomyces lilacinus, green fluorescent protein, fluorescence expression, transformants.