Gender modulation of Ca2+ uptake in cardiac mitochondria

Abstract Background: Mitochondrial calcium overload is an important factor in defining ischemia/reperfusion injury. Since pre-menopausal women are relatively protected from ischemia and heart disease, we tested the hypothesis that gender differences alter Ca 2+ handling in rat cardiac mitochondria. Methods: Using cardiac mitochondria isolated from male, female, and ovariectomized Sprague-Dawley rats, we measured mitochondrial calcium transport, redox state, and membrane potential (Δψ m ) during exposure to a calcium bolus. Redox state was modulated using either succinate (S) or succinate and pyruvate (SP) as substrates. Results: Net Ca 2+ uptake rates were significantly lower in female than male mitochondria using SP, substrate conditions that resulted in a lower redox state (NADH/NAD + ). Inhibition of the mitochondrial transition pore (MTP) using cyclosporin A showed significantly lower net Ca 2+ uptake in both substrate solutions when mitochondria from female and ovariectomized animals were compared to males, a finding consistent with gender modulation of the mitochondrial uniporter. Blockade of the Ca 2+ uniporter by ruthenium red abolished gender or substrate solution differences in calcium release. While there were no significant differences in resting Δψ m , or Δψ m following Ca 2+ addition, 80% of female samples recovered from Ca 2+ -induced depolarization compared to 57% and 43% of male and ovariectomized animals, respectively. Conclusions: Mitochondria from female hearts have lower Ca 2+ uptake rates under physiologic substrate solutions (succinate/pyruvate) and are able to appropriately maintain ΔYm under conditions of high [Ca 2+ ]. These differences are consistent with gender modulation of the Ca 2+ uniporter and may be a mechanism by which female myocardium suffers less injury with ischemia/reperfusion.