Protein Imprinted with Cyclodextrin Pseudo polyrotaxanes as Pseudo-supports

Abstract Protein molecularly imprinted polymer (MIPs) was prepared with cyclodextrin pseudo-polyrotaxanes (CD-PPRs) as pseudo-supports, acrylamide as the monomer and N,N -methylene bisacrylamide as the cross-linker. Firstly, CD-PPRs were obtained with γ-cyclodextrin as hosts and poly(vinyl alcohol) as guests. Then CD-PPRs were modified with acryloyl chloride. Subsequently, the cp-MIP was prepared in the presence of the modified CD-PPRs (CD-PPRs-Ac) and metal ions with bovine serum albumin (BSA) as the template. The MIPs adsorption amount to the BSA template increased initially and then decreased with the increase of CD-PPRs. It was found that the adsorption capacity of MIPs reached maximum value when the ratio of CD-PPRs-Ac to AM was about 0.55. The adsorption maximum reached to 5.16 mg g −1 under the Cu 2+ conditions. Finally, the imprinted polymer was used to purify the template from the protein mixtures containing four (BSA, ovalbumin, soybean trypsine inhibitor and lysozyme) different proteins to evaluate the adsorption specificity of MIPs. The experiments showed that the recognition specificity was improved significantly and the adsorption capacity increases by 6 times with the introduction of CD-PPRs.