Analytical techniques for cell fractions: XIII. Rotor A-XVI, a plastic gradient-reorienting rotor for isolating nuclei☆

Abstract The A-XVI rotor is a simply constructed and easily operated device for making centrifugal separation of whole cells and the larger formed subcellular particles from homogenates. Nuclei were successfully isolated on a sedimentation-rate basis. Studies were made on the effects of reorientation on sucrose gradients. It was shown that, with proper control of acceleration, deceleration, and internal configuration, sucrose gradients can be reoriented without serious degradation.