Gene expression response of transcription factor OsBTF3 in rice to bacterial and fungal infection and signal molecule treatment revealed by quantitative real-time PCR analysis.

It had been previously shown that the OsBTF3 gene encoding the RNA polymerase B transcription factor 3 was up-regulated in the suspension-cultured cells of rice co-incubated with Xanthomonas oryzae pv.oryzae(Xoo),the bacterial blight pathogen of rice.To elucidate expression dynamics of OsBTF3 gene in planta,the transcripts in the leaves of 2-week-old rice seedlings inoculated with the virulent strains of X.oryzae pv.oryzae(Xoo),X.oryzae pv.oryzicola(Xooc) and Magnaporthe grisea(Mg),or treated with the chemical signal molecules including salicylic acid(SA),ethane(ETH),methyl jasmonic acid(MeJA) and abscisic acid(ABA),respectively,were quantified by real-time PCR assay.The OsBTF3 gene expression response was observed to be significantly induced by bacterial and fungal infection with Xoo,Xooc and Mg during the susceptible interactions.However,the differential induction of OsBTF3 by the signal molecules was found.OsBTF3 transcript was increased drastically with ABA,MeJA and ETH,weakly with SA.The results indicated that rice OsBTF3 gene was differentially expressed in response to pathogen infection and signal molecules,and was potentially involved in plant disease susceptibility response.