Xenotransplante em camundongos imunossuprimidos coletado por meio de ovariectomia unilateral total vídeolaparoscópica em ovelha

The aim of this study was to describe the technique of gonadal xenotransplantation in immunosuppressed mice from total unilateral laparoscopic ovariectomy (OVL) in sheep. Four sheep were used as donors of reproductive tissue in mice in the reproductive phase (n = 12) as receivers. Samples were collected by laparoscopic surgery, evaluating the time of surgery, observation of behavioral anti-painful responses, monitoring appetite, body condition score and locomotion for 10 days postoperatively. The xenografts were performed in the renal capsule of mice, as evaluated histologically 60 days after transplantation. The operation time was 18 ± 3 min, the technique presented OVL minimally invasive surgical complications without providing minimum discomfort and optimum recovery of the animals and can be used in adult and prepubertal sheep for collecting reproductive tissues for the conservation of gametes. Histology tissue xenotransplanteted was a viable tissue, with neovascularization and absence of necrotic foci, but follicles at different stages were not visualized. It was concluded that the OVL technique can be employed in sheep and adult prepubertal for recovering reproductive tissue for xenotransplantation. For xenotransplantation, it is suggested that the ovarian fragments selected contain a sufficient number of primordial follicles for the possible success of biotech.