Phytochemical analysis and invitro assays for antimicrobial activity of Pluchea lanceolata extract against multi drug resistant Vibrio cholerae

In the present study water samples from different areas of Kanpur were collected and isolated three Vibrio cholerae strains. The growth pattern of V. cholerae strains were observed on TCBS agar and 3%, 5% and 8% salt concentration. Mostly V. cholerae strains were tolerate at 3% to 5% salt concentration but in this study one strain can tolerate at 8% salt concentration. The multidrug resistant activities of these strains were examined by different antibiotics i.e. Kanamycin, Gentamicin, ampicillin, cefixime, streptomycin and oxacilline. Methanolic leaf extract of Pluchea lanceolata were analyzed by qualitative phytochemical methods to identified different secondary metabolites. The different concentration (100%, 50%, 25% and 12.5%) of methanolic extract of Pluchea lanceolata was used as a biological tool to resolve the antibiotic problem. P. lanceolata extract inhibited the growth of isolated strains of V. cholerae at all concentrations and zone of diameter increased with the increase of concentrations. This study revealed that the extract of P. lanceolata is a potent antibacterial drug in the treatment of V. Cholerae infection. It provided to cholera patient if found effective and non toxic through in vivo studies.