dicyclohexylcarbodiimide-in membranes by Isolation of mycoplasma

mycoplasmas.The sensitivity of mycoplasmas to osmoticlysis varies amongthevarious species (5-7). Thefailure of some mycoplasmas to undergo hypo-tonic lysis led investigators to use other tech-niques suchas sonic irradiation (4, 11), digitoninlysis (13), freeze-thaw cycles (2, 4), and loadingthe cells with high