Methylation-assisted bisulfite sequencing to simultaneously map 5fC and 5caC on a genome-wide scale for DNA demethylation analysis.

Methylation-assisted bisulfite sequencing (MAB-seq) enables direct genome-scale mapping of 5fC and 5caC at single-base resolution to quantify DNA demethylation. Reduced representation (RRMAB-seq) also provides increased coverage on CpG-rich regions to reduce cost.