Abstract 3878: Proteomic approach to identify biomarkers for invasive cervix cancer: a prospective pilot study:

Objectives: Cervical cancer is the second most common cancer of women worldwide. In this prospective study, we attempted to identify a set of serum biomarkers, which could identify patients who have invasive cervical cancer and possibly help monitor disease status (recurrence, remission). Methods: Luminex bead array was used to measure serum protein concentrations of 19 different, promising proteins in cervical cancer patients (n = 23) compared to women with precancerous lesions (CIN2-3) (n = 20), and patients with normal cervical cytology (n = 20) in this IRB approved prospective study. Cervical cancer (adenocarcinoma and squamous carcinoma) patients had blood samples drawn within 30 days of diagnosis and repeat blood samples taken on follow up visits after completing therapy. These results were correlated to clinical data and outcomes. Protein expression was confirmed by immunohistochemistry in patient samples. Results: CIN and controls have very similar levels of serum proteins. Six proteins (CA153, OPG, CEA, IGFBP7, IGFBP4, and MMP-7) had significantly different levels in cervical cancer patients compared to controls and CIN (p < 0.05) with Area Under the Curve values of 0.74, 0.77, 0.79, 0.83, 0.84, and 0.98 respectively. IGFBP4 had a sensitivity of 75% at 95% specificity for correctly identifying invasive disease. MMP-7 had a sensitivity of 92% at 95% specificity. MMP-7 was confirmed by IHC in patient specific cervical cancer tissue. Conclusions: Several serum proteins are significantly altered in cervical cancer patients compared to controls and CIN, and are worth studying for further clinical utility. MMP-7 may prove to be an important mediator of cervical cancer. Citation Format: David P. Mysona, Adam Pyrzak, Jennifer Allen, Ashok Sharma, Paul Weinberger, Bunja Rungruang, Jin-Xiong She, Sharad Ghamande. Proteomic approach to identify biomarkers for invasive cervix cancer: a prospective pilot study. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 3878.