STUDIES ON β-PAROTIN VII. ISOLATION AND NATURE OF A GLYCOPEPTIDE FROM DIGESTED β-PAROTIN

A glycopeptide fraction was isolated from a digest by proteolytic enzyme of β-parotin by cellulose column chromatography using a mixture of n-propanol, ethanol, acetic acid and water as solvent.The glycopeptide fraction obtained in 8, 15 and 24hrs. digestion had no effect on the number of peripheral blood leukocytes in rabbits. Mixture of carbohydrate-free peptides was active. Therefore, it was suggested that the carbohydrate component was not concerned with the leukocyte activity of β-parotin. The glycopeptide contained approximately 35% of carbohydrates and its peptide moiety had seven amino acids. N-terminal amino acids of the peptide moiety were aspartic acid and glycine, and its C-terminal residue was aspartic acid.