Detection by reversed-phase hplc of trace amounts of the α113His globin chain in a sheep homozygous for a quadruple α-globin gene chromosome

Abstract 1. 1. In sheep αα 113His and α D αα 113His globin gene haplotypes, the percentage gene efficiencies, from the 5' to the 3' end, are about 32-18 and 30-14-6, respectively. 2. 2. We previously found that in a αααα homozygote there may be a 1–2% α 113His chains, which however were difficult to resolve from ga 113Leu chains by CMC chromatography. 3. 3. We report here the experimental conditions which allowed a neat resolution of the 1% α 113His chain peak by RP-HPLC. 4. 4. This finding strongly suggests the occurrence of the α D ααα 113His haplotype where the percentage gene efficiencies are 30-(14-5)-1, supporting the existence of a gradient of decreasing expression in multiple α-globin gene haplotypes.