Upregulation of Low-Density Lipoprotein Receptor After Exposure to Transforming Growth Factor-β2 in Tenon's Capsule Fibroblasts from Patients with Glaucoma

Abstract Purpose: The aim of this study was to investigate the dynamic alteration of a low-density lipoprotein receptor (LDLr) and its binding and uptake of LDL after exposure to transforming growth factor-β2 (TGF-β2) in Tenon's capsule fibroblasts from glaucoma patients. Methods: Tenon's capsule fibroblasts obtained from patients who had undergone selective glaucoma surgery were cultured and stimulated with different concentrations (0.1–10 ng/mL) of TGF-β2 for 1, 3, 5, and 7 days. Expression of LDLr mRNA was detected with relative quantification real-time reverse transcription–polymerase chain reaction, and expression of the corresponding protein was demonstrated with western blot. The extent of binding and uptake of 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI)-labeled LDL by GTFs was also checked. Results: After exposure to TGF-β2, LDLr was upregulated at the mRNA and protein levels in a concentration-dependent and/or time-dependent manner with a peak at a concentration of 5...