Correlation of hepatitis B surface antigen levels with HBV DNA on antiviral therapy

S 21 ANNUAL CONFERENCE —2013 V ra lH ep a itis not with bilirubin values (P <0.02). In acute heaptitis B LSM values had similar trend 14.3 kPa at entry, 12 kPa at 15 days but 6 out of ten cases had elevated kPa values (9.3 kPa) at time of complete recovery and on follow up showed gradual decline to normal in 4 cases alongwith HbsAg clearance. 2 out 6 cases had elevated kPa values (8.2 and 8.1 kPa) after six months with normal LFT. There was significant correlation with ALT (P <0.004) INR (P < 0.005) values but not with serum bilirubin. Conclusion: Liver stiffness values in acute hepatitis should be seen in context to ALT and INR values. In acute Hepatitis B persistent high LSM values with normal LFT may indicate progress towards chronic hepatitis. Larger studies are required Corresponding author. Punil Mehrotra. E-mail: punmel@yahoo.com ROLE OF THE BYSTANDER LIVER TISSUE IN DOCUMENTING OCCULT HEPATITIS B IN SURGICAL BIOPSY MATERIAL Kim Vaiphei Rajesh Kumar Department of Histopathology, Post Graduate Institue of Medical Education and Researc, Chandigarh, India Background: Hepatitis B virus (HBV) is important cause of chronic liver disease and one third world population is infected. A poorly recognised entity Occult HBV is presence of HBV DNA in liver or in serum in absence of detectable HBsAg in serum by current detectable assay. Objective: to study liver histology in biopsies detected to be HBV positive in patients with serology negative for HBsAg. Case selection criteria: Cases included in the study were those who were negative for serum HBsAg with no obvious clinical features of hepatitis and liver tissue was incidentally detected to be positive for HBV surface or/and core antigens by immunohistochemistry. Methods: Liver tissue obtained in resected specimen or in wedge biopsy in non-hepatitic conditions like extra-hepatic portal vein obstruction (EHPVO), hypersplenism, tumor metastasis, biliary obstruction or chronic cholecystitis. Sections bearing liver tissue were available for the study. Routine staining and immunohistochemistry were performed in all cases. Result: Forty three cases were studied.M: F=22:21,mean age inmale 43.3 and in female 40 years. All cases were positive for cytoplasmic HBcAg, 10 also showed nuclear HBcAg and 12 for HBsAg. Histological features: normal in 7, chronic hepatitis with activity in 22, metastasis in 14, changes consistent with EHPVO, 3 each of Budd Chiary and cholangitis, and 1 each of NCPF, HCC, granuloma and VOD. Histological features included steatosis, steatohepatitis, portal tract fibrosis with bridging, variable degree of portal tract inflammation, S64 interface hepatitis, lobular inflammation, cholangitis, angioma like portal vein and periductal fibrosis. Conclusion:Occult HBV cases exhibiting positivity for HB surface and/or core proteins with histological features of hepatitis is not a rare phenomenon. Hence, the present study further reiterates the statement of the noblest sample to document infection by hepatotrophic virus being the liver tissue itself. Corresponding author. Kim Vaiphei. E-mail: kvaiphei2009@gmail.com IL-28B GENOMIC POLYMORPHISM AS PREDICTOR OF TREATMENT RESPONSE TO INTERFERON BASED THERAPY FOR CHRONIC HCV INFECTIONEXPERIENCE FROM TERTIARY REFERRAL CENTRE FROM SOUTH INDIA K. A. Anoob John, S. Anil Kumar, P. Pradeep Kumar, Abhilash Haridas, N. Premletha, Vinayakumar Kattoor Ramakrishnan Department of Gastroenterology, Govt Medical College Trivandrum,