Clenbuterol detection operating fluid and detection method thereof

The invention discloses a clenbuterol detection operating fluid and a method thereof. The operating fluid is a colloidal solution obtained through mixing nano-gold colloid with a melamine solution, wherein the colloid particle size of the nano-gold colloid is smaller than 8nm; and the concentration of the melamine solution is 5[mu]M. The operating fluid combining the clenbuterol and melamine synergistically induced nano-gold aggregation effect and the catalytic activity of nano-gold mimic enzyme, and can be used to detect clenbuterol in a sample. A mimic enzyme chromogenic substrate involved in the invention is TMB, and the involved sample contains urine and pork. The clenbuterol visualization detection method has the advantages of high sensitivity, low cost and very good application prospect, and provides necessary support for future researches, production and supervision.