Isolation and properties of a highly purified Clostridium botulinum type F toxin

: A scheme for isolation of highly purified type F Cl. botulinum toxin has been developed. The scheme allows one to obtain the toxin with specific activity of 1.5--4.0 X 10(7) Dlm/mg protein, molecular weight of 150,000 daltons, a typical protein UV spectrum and the optical density ratio E280/E260 = 1.98.